All transcripts were created with artificial intelligence software and modified with manual review by a third party. Although we make every effort to ensure accuracy with the manual review, some may contain computer-generated mistranslations resulting in inaccurate or nonsensical word combinations, or unintentional language. FASEB and the presenting speakers did not review the transcripts and are not responsible and will not be held liable for damages, financial or otherwise, that occur as a result of transcript inaccuracies.
Growing Fat: Real-Time Visualization of Lipid Droplet Biogenesis Using Model Membranes
Raunaq Deo and William Prinz
Dept. of Cell Biology, UT Southwestern Medical Center, Dallas, TX
Lipid droplets (LDs) are storage depots of neutral lipids, found in all cells. They contain a core of neutral lipids surrounded by a monolayer of phospholipids. These unique structures are usually associated with the endoplasmic reticulum (ER) membrane where enzymes that produce neutral lipids like triacylglycerol (TG) and cholesterol ester (CE) are localized. How neutral lipids accumulate; forming LDs, leading to their growth and emergence is unclear. Using in vitro reconstitution, we have been able to selectively induce TG synthesis and recreate LD biogenesis on supported lipid bilayers. Using fluorescence microscopy, we directly follow de novo synthesis of LDs. Such a bottom-to-top reconstitution allows us to identify and modulate parameters such as membrane topology, spontaneous membrane curvature, line tension and surface tension which influence LD biogenesis. Furthermore, we are now investigating how LD-associated integral and peripheral membrane proteins (seipin and perilipins) may specifically regulate LD nucleation and growth.
Speakers
Raunaq Deo